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doi: 10.1016/j.obpill.2022.100047
We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol
PMCID: PMC7489598
"Short-term sleep deprivation leads to decreased systemic redox metabolites and altered epigenetic status." PLoS One
Without enough glutathione, toxins can "back up" in the liver, leading to the cellular stress that raises enzyme levels
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